Molecular detection of host cytokine expression in Helicobacter pylori infected patients via semi-quantitative RT-PCR
نویسندگان
چکیده
منابع مشابه
Detection of Helicobacter Pylori Diagnostic Antigens in the Stool of Infected Patients
Background: Some antigens of H. pylori are excreted into the stool of infected people. These antigens can be used to detect the infection by immunoassays such as ELISA. Our aim was to identify these antigens by immunoblotting and affinity chromatography techniques. Methods: Four different antigenic preparations, namely, whole cell sonicate (WCS), outer membrane proteins (OMPs), cytoplasmic anti...
متن کاملExpression of MicroRNA in Host Cells Infected with Helicobacter pylori
Background/Aims MicroRNAs (miRNAs) regulate gene expression. We assess miRNA regulation by Helicobacter pylori infection and elucidate their role in H. pylori-infected gastric epithelial cells. Methods The relationship between miRNA expression and DNA methylation was examined. Cells were treated with the nuclear factor-kappaB (NF-κB) inhibitor Bay 11-7082 to determine the relationship between...
متن کاملReal-time quantitative PCR for detection of Helicobacter pylori.
Helicobacter pylori is one of the most common chronic infections in humans, in whom it is a key etiological factor in peptic ulcer disease, gastric mucosa-associated lymphoid tissue lymphoma, and gastric adenocarcinoma. Humans are the bacterium's only host. Here we report the development of a real-time quantitative (Q) PCR-based assay to measure ureC gene copy number to detect H. pylori, based ...
متن کاملMolecular Detection of Oral Helicobacter pylori and Its Association with Dental Conditions Among Patients with Helicobacter Infection in Baghdad City
Background and Objective: Helicobacter pylori (H. pylori) is a bacterium known for its presence in the stomach, but it can also colonize the oral cavity and contribute to various oral and gastric diseases. This study aimed to detect the presence of oral H. pylori and investigate its potential association with dental caries and dental erosion. Methods: Saliva and plaque samples were collected f...
متن کاملCytokine expression in pediatric Helicobacter pylori infection.
Helicobacter pylori infection is one of the most common gastrointestinal infections worldwide and almost invariably causes chronic gastritis in the infected host. A predominant Th1 profile has been demonstrated in H. pylori-infected mucosa from adults, but no previous study has evaluated in situ cytokine expression in children. We therefore examined expression of proinflammatory, anti-inflammat...
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ژورنال
عنوان ژورنال: Indian Journal of Medical Microbiology
سال: 2010
ISSN: 0255-0857
DOI: 10.4103/0255-0857.58727